Size exclusion chromatography :
Subdivided into
1 - gel filtration
2 – gel permeation chromatography
Use aqueous solvents and hydrophilic packings.
nonpolar organic solvents and hydrophobic packings .
Packing for size-exclusion chromatography consists of small silica or polymer particles containing a network of uniform pores into which solute and solvent molecules can diffuse .
Advantage of Size-Exclusion Chromatography
1 - Short separation time
2 - Good sensitivity
3 - Freedom from sample loss
4 - Absence of column deactivation
No comments:
Post a Comment